ABSTRACT
Introdução: a espécie vegetal Curatella americana produz anualmente inflorescências com aroma adocicado rica em óleo essencial. Objetivo: avaliar as características físico-químicas, e atividades antifúngica e antioxidante do óleo essencial da flor de Curatella americana. Metodologia: as flores foram coletadas em quatro áreas de Cerrado no estado de Goiás; o rendimento de óleo essencial foi obtido através de hidrodestilação; as características físicas foram determinadas para densidade e solubilidade, a atividade antioxidante foi determinada pela redução do radical livre DPPH; a atividade antifúngica foi determinada por inibição das cepas de Candida, Sclerotinia sclerotiorum, Colletotrichum gloeosporioides e Aspergillus flavus. Resultados: o rendimento de óleo foi de 0,18%, densidade de 0,907 g mL-1, solubilidade positiva para EtOH 70%, atividade antioxidante de CI50 µL mL-1 1,95. Atividade de inibição fúngica apenas para Candida tropicalis na concentração de 8% com halo de antibiose de 10 mm. Sensibilidade discreta nas maiores concentrações de 25, 50 e 100 µL-1 para Aspergillus flavus e Sclerotinia sclerotiorum e baixa atividade de inibição para Colletotrichum gloeosporioides. Conclusão: o óleo essencial da flor de Curatella americana apresentou baixo rendimento, entretanto, alta eficiência na redução do radical livre DPPH. As atividades antifúngicas apresentaram bons resultados de inibição, entretanto, torna-se necessário a adição de outros óleos essenciais para aumento das taxas de inibição micelial.
Introduction: the plant species Curatella americana produces annual inflorescences with a sweet flavour rich in essential oil. Objective: to evaluate the physicochemical characteristics, antifungal and antioxidant activities of the essential oil of the Curatella americana flower. Methodology: the flowers were collected in four areas of Cerrado in the state of Goiás; the essential oil yield was obtained through hydrodistillation; the physical characteristics were determined for density and solubility, the antioxidant activity was determined by the reduction of the free radical DPPH; antifungal activity was determined by inhibiting the strains of Candida, Sclerotinia sclerotiorum, Colletotrichum gloeosporioides and Aspergillus flavus. Results: the oil yield was 0.18%, density 0.907 g mL-1, positive solubility for EtOH 70%, antioxidant activity of IC50 µL mL-1 1.95. Fungal inhibition activity only for Candida tropicalis at a concentration of 8% with a 10 mm antibiosis halo. Discrete sensitivity in the highest concentrations of 25, 50 and 100 µL-1 for Aspergillus flavus and Sclerotinia sclerotiorum and low inhibition activity for Colletotrichum gloeosporioides. Conclusion: The essential oil of the Curatella americana flower showed low yield, however, high efficiency in reducing DPPH free radical. Antifungal activities showed good inhibition results, however, it is necessary to add other essential oils to increase mycelial inhibition rates.
Subject(s)
Candidiasis , Oils, Volatile , Aflatoxins , Flowers , DilleniaceaeABSTRACT
RESUMEN Objetivo: determinar la prevalencia de vaginosis bacteriana (VB), candidiasis, tricomoniasis y sus factores asociados, en las mujeres que consultan al servicio de citología de dos empresas sociales del estado (ESEs) de los municipios de Apartadó y Rionegro, año 2014. Metodología: estudio descriptivo de corte transversal. Fuente de información secundaria: base de datos del Laboratorio de Citología de la Escuela de Microbiología. Población: 6447 registros de mujeres del servicio de citología de los municipios de Apartadó y Rionegro. 3.762 registros de Apartadó y 2.685 registros de Rionegro año 2014. Se calcularon frecuencias absolutas, relativas e intervalos de confianza. Se exploró la asociación entre las variables independientes con relación significativa con los microorganismos estudiados, luego se ingresaron a un modelo de ajuste multivariado de regresión logística binaria. Nivel de significación estadística del 0,05. Los datos se almacenaron y analizaron en SPSS 20,1®. Resultados: la prevalencia de vaginosis bacteriana fue 29,9 %, candidiasis del 10,7 %, y tricomoniasis del 3,2 %. No hubo diferencias significativas en las infecciones en ambos municipios. No se encontró asociación estadística significativa con los factores de riesgo estudiados. Conclusiones: el comportamiento de las VB, cándida y trichomona fue muy similar al reportado en otras investigaciones y no se encontró diferencia en el comportamiento en los municipios estudiados, a pesar de ser contextos socioeconómicos y culturales diferentes.
SUMMARY Objective: To determine the prevalence of bacterial vaginosis, candidiasis, trichomoniasis, and associated factors in women who use the cytology service of two hospitals in Apartadó and Rionegro in 2014. Methodology: Descriptive cross-sectional study. Data source: the cytology laboratory database of the School of Microbiology. Study population: 6447 samples from women who attended the cytology service in Apartadó hospital with 3,762 records and Rionegro hospital with 2,685 records. Absolute and relative frequencies with confidence intervals were calculated, the association between the independent variables that showed significant association with the microorganisms studied were explored, and then, entered in a multivariate binary logistic regression model. In all analyzes statistical significance level of 0.05 was accepted and data were stored and analyzed with SPSS 20,1®. Results: The prevalence of bacterial vaginosis was 29.9 %, candidiasis 10.7% and trichomoniasis 3.2 %. No significant differences between municipalities were observed nor statistically significant association with any of the risk factors studied. Conclusions: The behavior of BV, candida and trichomona were very similar to those reported in other researches and no difference was found between the studied municipalities, despite the differences in socioeconomic and cultural contexts.